Read the following passage on transport, and mark the letter A, B, C, or D on your answer sheet to indicate the correct answer to each of the questions from 38 to 42.

(1) The protozoans, minute, aquatic creatures each of which consists of a single cell of protoplasm, constitute a classification of the most primitive forms of animal life. They are fantastically diverse, but three major groups may be identified on the basis of their motility. The Mastigophora have one or more long tails, which they use to project themselves forward. The Ciliata, which use the same basic means for locomotion as the Mastigophora, have a larger number of short tails. The Sarcodina, which include amoebae, float or row themselves about on their crusted bodies.

(2) In addition to their form of movement, several other features discriminate among the three groups of protozoans. For example, at least two nuclei per cell have been identified in the Ciliata, usually a large nucleus that regulates growth but decomposes during reproduction, and a smaller one that contains the genetic code necessary to generate the large nucleus.

(3) Protozoans are considered animals because, unlike pigmented plants to which some protozoans are otherwise almost identical, they do not live on simple organic compounds. Their cell demonstrates all of the major characteristics of the cells of higher animals.

(4) Many species of protozoans collect into colonies, physically connected to each other and responding uniformly to outside stimulae. Current research into this phenomenon, along with investigations carried out with advanced microscopes may necessitate a redefinition of what constitutes protozoans, even calling into question the basic premise that they have only one cell. Nevertheless, with the current data available, almost 40,000 species of protozoans have been identified. No doubt, as the technology improves our methods of observation, better models of classification will be proposed.

With what topic is the passage primarily concerned?

He _________ alone a month ago, and _________ of since.

He _ alone a month ago, and _ of since.